il5 (R&D Systems)
Structured Review

Il5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+il5/Mouse+IL-5+DuoSet+ELISA/pm40524528-222-15-24
Average 95 stars, based on 58 article reviews
Images
1) Product Images from "Role for Complement C5 in Eosinophilic Inflammation of Severe Asthma."
Article Title: Role for Complement C5 in Eosinophilic Inflammation of Severe Asthma.
Journal: Allergy
doi: 10.1111/all.16616
Figure Legend Snippet: FIGURE 6 | Complement C5 alone promotes eosinophil recruitment and activation in IL-5 transgenic mouse. (a) Experimental scheme of daily challenge of an IL-5 transgenic mouse (IL5-tg) with recombinant mouse C5. (b) Change in baseline airway resistance during acetylcholine airway challenge in IL5-tg mice treated with either phosphate-buffered saline (PBS) or Complement C5. (c) Bronchoalveolar lavage fluid (BALF) eosinophil percentage (CD45+live+CD11c−SiglecF+). (d) BALF eosinophil cell count (e, f) BALF eosinophil granule proteins of mouse eosinophil peroxidase (EPX) and mouse eosinophil cationic protein ECP (RNASE3) levels in IL5-tg mice treated with phosphate-buffered saline (PBS) or Complement C5. (g) Peribronchial inflammatory infiltration and inflammatory score, (h) Periodic Acid-Schiff (PAS) staining for epithelial goblet cell metaplasia and PAS positive cells per bronchi. Data show mean ± SD. Data was analyzed with one-way analysis of variance (ANOVA) with Tukey's post hoc test. **p < 0.01. N = 5 each group, all experiments were performed by different operators and repeated twice. The data presented in the main figures represent the results from one batch of experiments, which consistently showed similar trends across the repetitions.
Techniques Used: Activation Assay, Transgenic Assay, Recombinant, Saline, Cell Counting, Staining
Related Articles
other:Article Title: Obesity alters the lung myeloid cell landscape to enhance breast cancer metastasis through IL5 and GM-CSF Article Snippet: An Eppendorf Plate Reader AF2200 was used for colorimetric quantification and analysis at 460 nm and 570 nm wavelengths. Enzyme-linked Immunosorbent Assay:Article Title: Obesity alters the lung myeloid cell landscape to enhance breast cancer metastasis through IL5 and GM-CSF Article Snippet: .. Human and TUNEL Assay:Article Title: Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils. Article Snippet: .. Aortic SMC Apoptosis and TUNEL Staining: Mouse aortic SMCs were plated onto an 8-well chamber slide and pretreated with or without EOS lysates from WT mice at 1 × 106 EOS mL−1, ILC2 lysates from WT and Il5−/− mice at 2 × 104 ILC2 mL−1, and 10 ng mL−1 Staining:Article Title: Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils. Article Snippet: .. Aortic SMC Apoptosis and TUNEL Staining: Mouse aortic SMCs were plated onto an 8-well chamber slide and pretreated with or without EOS lysates from WT mice at 1 × 106 EOS mL−1, ILC2 lysates from WT and Il5−/− mice at 2 × 104 ILC2 mL−1, and 10 ng mL−1 Incubation:Article Title: Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils. Article Snippet: .. Aortic SMC Apoptosis and TUNEL Staining: Mouse aortic SMCs were plated onto an 8-well chamber slide and pretreated with or without EOS lysates from WT mice at 1 × 106 EOS mL−1, ILC2 lysates from WT and Il5−/− mice at 2 × 104 ILC2 mL−1, and 10 ng mL−1 Article Title: Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils Article Snippet: .. Mouse aortic SMCs were plated onto an 8‐well chamber slide and pretreated with or without EOS lysates from WT mice at 1 × 10 6 EOS mL −1 , ILC2 lysates from WT and Il5 −/− mice at 2 × 10 4 ILC2 mL −1 , and 10 ng mL −1 |
