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il5  (R&D Systems)


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    Structured Review

    R&D Systems il5
    FIGURE 6 | Complement C5 alone promotes eosinophil recruitment and activation in IL-5 transgenic mouse. (a) Experimental scheme of daily challenge of an IL-5 transgenic mouse <t>(IL5-tg)</t> with recombinant mouse C5. (b) Change in baseline airway resistance during acetylcholine airway challenge in IL5-tg mice treated with either phosphate-buffered saline (PBS) or Complement C5. (c) Bronchoalveolar lavage fluid (BALF) eosinophil percentage (CD45+live+CD11c−SiglecF+). (d) BALF eosinophil cell count (e, f) BALF eosinophil granule proteins of mouse eosinophil peroxidase (EPX) and mouse eosinophil cationic protein ECP (RNASE3) levels in IL5-tg mice treated with phosphate-buffered saline (PBS) or Complement C5. (g) Peribronchial inflammatory infiltration and inflammatory score, (h) Periodic Acid-Schiff (PAS) staining for epithelial goblet cell metaplasia and PAS positive cells per bronchi. Data show mean ± SD. Data was analyzed with one-way analysis of variance (ANOVA) with Tukey's post hoc test. **p < 0.01. N = 5 each group, all experiments were performed by different operators and repeated twice. The data presented in the main figures represent the results from one batch of experiments, which consistently showed similar trends across the repetitions.
    Il5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il5/Mouse+IL-5+DuoSet+ELISA/pm40524528-222-15-24
    Average 95 stars, based on 58 article reviews
    il5 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Role for Complement C5 in Eosinophilic Inflammation of Severe Asthma."

    Article Title: Role for Complement C5 in Eosinophilic Inflammation of Severe Asthma.

    Journal: Allergy

    doi: 10.1111/all.16616

    FIGURE 6 | Complement C5 alone promotes eosinophil recruitment and activation in IL-5 transgenic mouse. (a) Experimental scheme of daily challenge of an IL-5 transgenic mouse (IL5-tg) with recombinant mouse C5. (b) Change in baseline airway resistance during acetylcholine airway challenge in IL5-tg mice treated with either phosphate-buffered saline (PBS) or Complement C5. (c) Bronchoalveolar lavage fluid (BALF) eosinophil percentage (CD45+live+CD11c−SiglecF+). (d) BALF eosinophil cell count (e, f) BALF eosinophil granule proteins of mouse eosinophil peroxidase (EPX) and mouse eosinophil cationic protein ECP (RNASE3) levels in IL5-tg mice treated with phosphate-buffered saline (PBS) or Complement C5. (g) Peribronchial inflammatory infiltration and inflammatory score, (h) Periodic Acid-Schiff (PAS) staining for epithelial goblet cell metaplasia and PAS positive cells per bronchi. Data show mean ± SD. Data was analyzed with one-way analysis of variance (ANOVA) with Tukey's post hoc test. **p < 0.01. N = 5 each group, all experiments were performed by different operators and repeated twice. The data presented in the main figures represent the results from one batch of experiments, which consistently showed similar trends across the repetitions.
    Figure Legend Snippet: FIGURE 6 | Complement C5 alone promotes eosinophil recruitment and activation in IL-5 transgenic mouse. (a) Experimental scheme of daily challenge of an IL-5 transgenic mouse (IL5-tg) with recombinant mouse C5. (b) Change in baseline airway resistance during acetylcholine airway challenge in IL5-tg mice treated with either phosphate-buffered saline (PBS) or Complement C5. (c) Bronchoalveolar lavage fluid (BALF) eosinophil percentage (CD45+live+CD11c−SiglecF+). (d) BALF eosinophil cell count (e, f) BALF eosinophil granule proteins of mouse eosinophil peroxidase (EPX) and mouse eosinophil cationic protein ECP (RNASE3) levels in IL5-tg mice treated with phosphate-buffered saline (PBS) or Complement C5. (g) Peribronchial inflammatory infiltration and inflammatory score, (h) Periodic Acid-Schiff (PAS) staining for epithelial goblet cell metaplasia and PAS positive cells per bronchi. Data show mean ± SD. Data was analyzed with one-way analysis of variance (ANOVA) with Tukey's post hoc test. **p < 0.01. N = 5 each group, all experiments were performed by different operators and repeated twice. The data presented in the main figures represent the results from one batch of experiments, which consistently showed similar trends across the repetitions.

    Techniques Used: Activation Assay, Transgenic Assay, Recombinant, Saline, Cell Counting, Staining

    Related Articles

    other:

    Article Title: Obesity alters the lung myeloid cell landscape to enhance breast cancer metastasis through IL5 and GM-CSF
    Article Snippet: An Eppendorf Plate Reader AF2200 was used for colorimetric quantification and analysis at 460 nm and 570 nm wavelengths.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Obesity alters the lung myeloid cell landscape to enhance breast cancer metastasis through IL5 and GM-CSF
    Article Snippet: .. Human and Mouse IL5 and GM-CSF Quantikine ELISA kits were obtained from R&D Systems (catalogue no.: Human IL5 no. D5000B; human GM-CSF no. DGM00; mouse IL5 no. M5000; mouse GM-CSF no. MGM00), and ELISAs were performed according to the manufacturer’s protocol. .. An Eppendorf Plate Reader AF2200 was used for colorimetric quantification and analysis at 460 nm and 570 nm wavelengths.

    TUNEL Assay:

    Article Title: Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.
    Article Snippet: .. Aortic SMC Apoptosis and TUNEL Staining: Mouse aortic SMCs were plated onto an 8-well chamber slide and pretreated with or without EOS lysates from WT mice at 1 × 106 EOS mL−1, ILC2 lysates from WT and Il5−/− mice at 2 × 104 ILC2 mL−1, and 10 ng mL−1 mouse IL5 (405-ML, R&D Systems) for 48 h. Then SMCs were incubated with or without 100 × 10−6 m PDTC (P8765, Sigma-Aldrich) for 5 h to induce apoptosis. .. Apoptotic SMCs were fixed with methanol for 1 min and assessed with the insitu apoptosis detection kit (S7100, EMD Millipore).

    Staining:

    Article Title: Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.
    Article Snippet: .. Aortic SMC Apoptosis and TUNEL Staining: Mouse aortic SMCs were plated onto an 8-well chamber slide and pretreated with or without EOS lysates from WT mice at 1 × 106 EOS mL−1, ILC2 lysates from WT and Il5−/− mice at 2 × 104 ILC2 mL−1, and 10 ng mL−1 mouse IL5 (405-ML, R&D Systems) for 48 h. Then SMCs were incubated with or without 100 × 10−6 m PDTC (P8765, Sigma-Aldrich) for 5 h to induce apoptosis. .. Apoptotic SMCs were fixed with methanol for 1 min and assessed with the insitu apoptosis detection kit (S7100, EMD Millipore).

    Incubation:

    Article Title: Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.
    Article Snippet: .. Aortic SMC Apoptosis and TUNEL Staining: Mouse aortic SMCs were plated onto an 8-well chamber slide and pretreated with or without EOS lysates from WT mice at 1 × 106 EOS mL−1, ILC2 lysates from WT and Il5−/− mice at 2 × 104 ILC2 mL−1, and 10 ng mL−1 mouse IL5 (405-ML, R&D Systems) for 48 h. Then SMCs were incubated with or without 100 × 10−6 m PDTC (P8765, Sigma-Aldrich) for 5 h to induce apoptosis. .. Apoptotic SMCs were fixed with methanol for 1 min and assessed with the insitu apoptosis detection kit (S7100, EMD Millipore).

    Article Title: Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils
    Article Snippet: .. Mouse aortic SMCs were plated onto an 8‐well chamber slide and pretreated with or without EOS lysates from WT mice at 1 × 10 6 EOS mL −1 , ILC2 lysates from WT and Il5 −/− mice at 2 × 10 4 ILC2 mL −1 , and 10 ng mL −1 mouse IL5 (405‐ML, R&D Systems) for 48 h. Then SMCs were incubated with or without 100 × 10 −6 m PDTC (P8765, Sigma‐Aldrich) for 5 h to induce apoptosis. .. Apoptotic SMCs were fixed with methanol for 1 min and assessed with the in‐situ apoptosis detection kit (S7100, EMD Millipore).



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    FIGURE 6 | Complement C5 alone promotes eosinophil recruitment and activation in IL-5 transgenic mouse. (a) Experimental scheme of daily challenge of an IL-5 transgenic mouse <t>(IL5-tg)</t> with recombinant mouse C5. (b) Change in baseline airway resistance during acetylcholine airway challenge in IL5-tg mice treated with either phosphate-buffered saline (PBS) or Complement C5. (c) Bronchoalveolar lavage fluid (BALF) eosinophil percentage (CD45+live+CD11c−SiglecF+). (d) BALF eosinophil cell count (e, f) BALF eosinophil granule proteins of mouse eosinophil peroxidase (EPX) and mouse eosinophil cationic protein ECP (RNASE3) levels in IL5-tg mice treated with phosphate-buffered saline (PBS) or Complement C5. (g) Peribronchial inflammatory infiltration and inflammatory score, (h) Periodic Acid-Schiff (PAS) staining for epithelial goblet cell metaplasia and PAS positive cells per bronchi. Data show mean ± SD. Data was analyzed with one-way analysis of variance (ANOVA) with Tukey's post hoc test. **p < 0.01. N = 5 each group, all experiments were performed by different operators and repeated twice. The data presented in the main figures represent the results from one batch of experiments, which consistently showed similar trends across the repetitions.
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    FIGURE 6 | Complement C5 alone promotes eosinophil recruitment and activation in IL-5 transgenic mouse. (a) Experimental scheme of daily challenge of an IL-5 transgenic mouse <t>(IL5-tg)</t> with recombinant mouse C5. (b) Change in baseline airway resistance during acetylcholine airway challenge in IL5-tg mice treated with either phosphate-buffered saline (PBS) or Complement C5. (c) Bronchoalveolar lavage fluid (BALF) eosinophil percentage (CD45+live+CD11c−SiglecF+). (d) BALF eosinophil cell count (e, f) BALF eosinophil granule proteins of mouse eosinophil peroxidase (EPX) and mouse eosinophil cationic protein ECP (RNASE3) levels in IL5-tg mice treated with phosphate-buffered saline (PBS) or Complement C5. (g) Peribronchial inflammatory infiltration and inflammatory score, (h) Periodic Acid-Schiff (PAS) staining for epithelial goblet cell metaplasia and PAS positive cells per bronchi. Data show mean ± SD. Data was analyzed with one-way analysis of variance (ANOVA) with Tukey's post hoc test. **p < 0.01. N = 5 each group, all experiments were performed by different operators and repeated twice. The data presented in the main figures represent the results from one batch of experiments, which consistently showed similar trends across the repetitions.
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    FIGURE 6 | Complement C5 alone promotes eosinophil recruitment and activation in IL-5 transgenic mouse. (a) Experimental scheme of daily challenge of an IL-5 transgenic mouse <t>(IL5-tg)</t> with recombinant mouse C5. (b) Change in baseline airway resistance during acetylcholine airway challenge in IL5-tg mice treated with either phosphate-buffered saline (PBS) or Complement C5. (c) Bronchoalveolar lavage fluid (BALF) eosinophil percentage (CD45+live+CD11c−SiglecF+). (d) BALF eosinophil cell count (e, f) BALF eosinophil granule proteins of mouse eosinophil peroxidase (EPX) and mouse eosinophil cationic protein ECP (RNASE3) levels in IL5-tg mice treated with phosphate-buffered saline (PBS) or Complement C5. (g) Peribronchial inflammatory infiltration and inflammatory score, (h) Periodic Acid-Schiff (PAS) staining for epithelial goblet cell metaplasia and PAS positive cells per bronchi. Data show mean ± SD. Data was analyzed with one-way analysis of variance (ANOVA) with Tukey's post hoc test. **p < 0.01. N = 5 each group, all experiments were performed by different operators and repeated twice. The data presented in the main figures represent the results from one batch of experiments, which consistently showed similar trends across the repetitions.
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    Image Search Results


    FIGURE 6 | Complement C5 alone promotes eosinophil recruitment and activation in IL-5 transgenic mouse. (a) Experimental scheme of daily challenge of an IL-5 transgenic mouse (IL5-tg) with recombinant mouse C5. (b) Change in baseline airway resistance during acetylcholine airway challenge in IL5-tg mice treated with either phosphate-buffered saline (PBS) or Complement C5. (c) Bronchoalveolar lavage fluid (BALF) eosinophil percentage (CD45+live+CD11c−SiglecF+). (d) BALF eosinophil cell count (e, f) BALF eosinophil granule proteins of mouse eosinophil peroxidase (EPX) and mouse eosinophil cationic protein ECP (RNASE3) levels in IL5-tg mice treated with phosphate-buffered saline (PBS) or Complement C5. (g) Peribronchial inflammatory infiltration and inflammatory score, (h) Periodic Acid-Schiff (PAS) staining for epithelial goblet cell metaplasia and PAS positive cells per bronchi. Data show mean ± SD. Data was analyzed with one-way analysis of variance (ANOVA) with Tukey's post hoc test. **p < 0.01. N = 5 each group, all experiments were performed by different operators and repeated twice. The data presented in the main figures represent the results from one batch of experiments, which consistently showed similar trends across the repetitions.

    Journal: Allergy

    Article Title: Role for Complement C5 in Eosinophilic Inflammation of Severe Asthma.

    doi: 10.1111/all.16616

    Figure Lengend Snippet: FIGURE 6 | Complement C5 alone promotes eosinophil recruitment and activation in IL-5 transgenic mouse. (a) Experimental scheme of daily challenge of an IL-5 transgenic mouse (IL5-tg) with recombinant mouse C5. (b) Change in baseline airway resistance during acetylcholine airway challenge in IL5-tg mice treated with either phosphate-buffered saline (PBS) or Complement C5. (c) Bronchoalveolar lavage fluid (BALF) eosinophil percentage (CD45+live+CD11c−SiglecF+). (d) BALF eosinophil cell count (e, f) BALF eosinophil granule proteins of mouse eosinophil peroxidase (EPX) and mouse eosinophil cationic protein ECP (RNASE3) levels in IL5-tg mice treated with phosphate-buffered saline (PBS) or Complement C5. (g) Peribronchial inflammatory infiltration and inflammatory score, (h) Periodic Acid-Schiff (PAS) staining for epithelial goblet cell metaplasia and PAS positive cells per bronchi. Data show mean ± SD. Data was analyzed with one-way analysis of variance (ANOVA) with Tukey's post hoc test. **p < 0.01. N = 5 each group, all experiments were performed by different operators and repeated twice. The data presented in the main figures represent the results from one batch of experiments, which consistently showed similar trends across the repetitions.

    Article Snippet: Mouse BALF concentrations of IL4 (Mouse IL- 4 DuoSet ELISA; Cat# DY404- 05, R&D SYSTEMS), IL5 (Mouse IL- 5 DuoSet ELISA; Cat# DY405- 05, R&D SYSTEMS), IL13 (Mouse IL- 13 DuoSet ELISA; Cat# DY413- 05, R&D SYSTEMS), IL- 17A (IL- 17 Mouse ELISA Kit; Cat# BMS6001TEN, Thermo Fisher), mouse eosinophil peroxidase (EPX) ELISA Kit (Cat# abx153959; Abbexa), and mouse eosinophil cationic protein/ ECP (RNASE3) ELISA Kit (Cat# abx053689; Abbexa) were measured by sandwich ELISA.

    Techniques: Activation Assay, Transgenic Assay, Recombinant, Saline, Cell Counting, Staining

    Journal: Cell reports

    Article Title: CB2 stimulation of adipose resident ILC2s orchestrates immune balance and ameliorates type 2 diabetes mellitus

    doi: 10.1016/j.celrep.2024.114434

    Figure Lengend Snippet:

    Article Snippet: Mouse: C57BL/6- Il5 tm1Kopf /J , Jackson laboratories , Cat # 003175.

    Techniques: Recombinant, Staining, Enzyme-linked Immunosorbent Assay, Software